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Gene expression analysis of chondrocytes and hypertrophic chondrocyte-related markers across experimental conditions. ( A ) Expression of chondrocyte-associated genes showing Col2A1 (type II collagen) with increased expression in P2ce compared to the other groups. ACAN (aggrecan) demonstrated significantly higher expression in P2ce versus. SOX9 expression was significantly increased in P2ce compared to Control. <t>COMP</t> showed significantly higher expression in P2ce versus free-swelling Control. ( B ) Expression of hypertrophic chondrocyte markers revealing Col10A1 (type X collagen) with no significant variations between the three loaded groups, but significant upregulation in P2ce and in P2te compared to free swelling. MMP13 was significantly elevated in P2ce and in P2te versus Control. RUNX2 showed increased expression in P2te compared to Control. In both cases, no significant differences were detected between the three loaded groups. ALP demonstrated significant upregulation in P2ce versus P1 and P2te versus Control. Data presented as log 10 fold-changes relative to baseline (dotted line at 1.0) except for Col2A1 and ACAN expressed as log 10 gene expression with individual donor points (average of technical replicates) overlaid on box plots showing median and quartiles (n = 6 per group). * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.
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Gene expression analysis of chondrocytes and hypertrophic chondrocyte-related markers across experimental conditions. ( A ) Expression of chondrocyte-associated genes showing Col2A1 (type II collagen) with increased expression in P2ce compared to the other groups. ACAN (aggrecan) demonstrated significantly higher expression in P2ce versus. SOX9 expression was significantly increased in P2ce compared to Control. <t>COMP</t> showed significantly higher expression in P2ce versus free-swelling Control. ( B ) Expression of hypertrophic chondrocyte markers revealing Col10A1 (type X collagen) with no significant variations between the three loaded groups, but significant upregulation in P2ce and in P2te compared to free swelling. MMP13 was significantly elevated in P2ce and in P2te versus Control. RUNX2 showed increased expression in P2te compared to Control. In both cases, no significant differences were detected between the three loaded groups. ALP demonstrated significant upregulation in P2ce versus P1 and P2te versus Control. Data presented as log 10 fold-changes relative to baseline (dotted line at 1.0) except for Col2A1 and ACAN expressed as log 10 gene expression with individual donor points (average of technical replicates) overlaid on box plots showing median and quartiles (n = 6 per group). * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.
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Gene expression analysis of chondrocytes and hypertrophic chondrocyte-related markers across experimental conditions. ( A ) Expression of chondrocyte-associated genes showing Col2A1 (type II collagen) with increased expression in P2ce compared to the other groups. ACAN (aggrecan) demonstrated significantly higher expression in P2ce versus. SOX9 expression was significantly increased in P2ce compared to Control. <t>COMP</t> showed significantly higher expression in P2ce versus free-swelling Control. ( B ) Expression of hypertrophic chondrocyte markers revealing Col10A1 (type X collagen) with no significant variations between the three loaded groups, but significant upregulation in P2ce and in P2te compared to free swelling. MMP13 was significantly elevated in P2ce and in P2te versus Control. RUNX2 showed increased expression in P2te compared to Control. In both cases, no significant differences were detected between the three loaded groups. ALP demonstrated significant upregulation in P2ce versus P1 and P2te versus Control. Data presented as log 10 fold-changes relative to baseline (dotted line at 1.0) except for Col2A1 and ACAN expressed as log 10 gene expression with individual donor points (average of technical replicates) overlaid on box plots showing median and quartiles (n = 6 per group). * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.
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Gene expression analysis of chondrocytes and hypertrophic chondrocyte-related markers across experimental conditions. ( A ) Expression of chondrocyte-associated genes showing Col2A1 (type II collagen) with increased expression in P2ce compared to the other groups. ACAN (aggrecan) demonstrated significantly higher expression in P2ce versus. SOX9 expression was significantly increased in P2ce compared to Control. COMP showed significantly higher expression in P2ce versus free-swelling Control. ( B ) Expression of hypertrophic chondrocyte markers revealing Col10A1 (type X collagen) with no significant variations between the three loaded groups, but significant upregulation in P2ce and in P2te compared to free swelling. MMP13 was significantly elevated in P2ce and in P2te versus Control. RUNX2 showed increased expression in P2te compared to Control. In both cases, no significant differences were detected between the three loaded groups. ALP demonstrated significant upregulation in P2ce versus P1 and P2te versus Control. Data presented as log 10 fold-changes relative to baseline (dotted line at 1.0) except for Col2A1 and ACAN expressed as log 10 gene expression with individual donor points (average of technical replicates) overlaid on box plots showing median and quartiles (n = 6 per group). * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.

Journal: Cells

Article Title: Transient Early Mechanical Loading Induces Hypertrophic Chondrocyte Differentiation of Human Mesenchymal Stromal Cells

doi: 10.3390/cells14221773

Figure Lengend Snippet: Gene expression analysis of chondrocytes and hypertrophic chondrocyte-related markers across experimental conditions. ( A ) Expression of chondrocyte-associated genes showing Col2A1 (type II collagen) with increased expression in P2ce compared to the other groups. ACAN (aggrecan) demonstrated significantly higher expression in P2ce versus. SOX9 expression was significantly increased in P2ce compared to Control. COMP showed significantly higher expression in P2ce versus free-swelling Control. ( B ) Expression of hypertrophic chondrocyte markers revealing Col10A1 (type X collagen) with no significant variations between the three loaded groups, but significant upregulation in P2ce and in P2te compared to free swelling. MMP13 was significantly elevated in P2ce and in P2te versus Control. RUNX2 showed increased expression in P2te compared to Control. In both cases, no significant differences were detected between the three loaded groups. ALP demonstrated significant upregulation in P2ce versus P1 and P2te versus Control. Data presented as log 10 fold-changes relative to baseline (dotted line at 1.0) except for Col2A1 and ACAN expressed as log 10 gene expression with individual donor points (average of technical replicates) overlaid on box plots showing median and quartiles (n = 6 per group). * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.

Article Snippet: COMP , Hs00164359_m1 , , .

Techniques: Gene Expression, Expressing, Control